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rh norrin  (R&D Systems)


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    R&D Systems rh norrin
    Expression of tag-less <t>Norrin</t> <t>K86P</t> in BL21(DE3) cell inclusion body protein fractions. DISC-PAGE protein gel of inclusion body (IB) preparations from four separate batches are shown. IB material was dissolved and reduced by heating (100 °C × 10 min) in SDS sample buffer with 2-mercaptoethanol. Some dimeric and tetrameric bands can be formed during this treatment. Two different loading volumes (1 µL and 5 µL) were used for each of the four batches shown. Gels were stained with colloidal Coomassie blue. IB protein content was >98% recombinant Norrin K86P .
    Rh Norrin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rh norrin/product/R&D Systems
    Average 93 stars, based on 8 article reviews
    rh norrin - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "Development of Norrin-Based Protein Therapeutic for Activation of Norrin-Wnt Signaling in Human Retinal Endothelial Cells"

    Article Title: Development of Norrin-Based Protein Therapeutic for Activation of Norrin-Wnt Signaling in Human Retinal Endothelial Cells

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms262311340

    Expression of tag-less Norrin K86P in BL21(DE3) cell inclusion body protein fractions. DISC-PAGE protein gel of inclusion body (IB) preparations from four separate batches are shown. IB material was dissolved and reduced by heating (100 °C × 10 min) in SDS sample buffer with 2-mercaptoethanol. Some dimeric and tetrameric bands can be formed during this treatment. Two different loading volumes (1 µL and 5 µL) were used for each of the four batches shown. Gels were stained with colloidal Coomassie blue. IB protein content was >98% recombinant Norrin K86P .
    Figure Legend Snippet: Expression of tag-less Norrin K86P in BL21(DE3) cell inclusion body protein fractions. DISC-PAGE protein gel of inclusion body (IB) preparations from four separate batches are shown. IB material was dissolved and reduced by heating (100 °C × 10 min) in SDS sample buffer with 2-mercaptoethanol. Some dimeric and tetrameric bands can be formed during this treatment. Two different loading volumes (1 µL and 5 µL) were used for each of the four batches shown. Gels were stained with colloidal Coomassie blue. IB protein content was >98% recombinant Norrin K86P .

    Techniques Used: Expressing, Staining, Recombinant

    Fundus and fluorescein angiography images before and after injection of Norrin K86P . Long Evans rats were imaged pre injection and 3 weeks post injection of 250 ng Norrin K86P . An example of the same Norin K86P -treated eye (OS) and vehicle-treated contralateral eye (OD) are shown. Brightfield fundus images are shown for each eye with their corresponding fluoresceine angiography image below. Angiography shows the perfused microvasculature of the neural retina (green). No impact on the retinal vasculature was noted for any eyes, whether Norin K86P - or vehicle-injected, across all rats tested. (N = 4). The same retinas were also analyzed for retinal thickness using SD-OCT.
    Figure Legend Snippet: Fundus and fluorescein angiography images before and after injection of Norrin K86P . Long Evans rats were imaged pre injection and 3 weeks post injection of 250 ng Norrin K86P . An example of the same Norin K86P -treated eye (OS) and vehicle-treated contralateral eye (OD) are shown. Brightfield fundus images are shown for each eye with their corresponding fluoresceine angiography image below. Angiography shows the perfused microvasculature of the neural retina (green). No impact on the retinal vasculature was noted for any eyes, whether Norin K86P - or vehicle-injected, across all rats tested. (N = 4). The same retinas were also analyzed for retinal thickness using SD-OCT.

    Techniques Used: Injection

    Retinal thickness before and after intraocular injection of Norrin K86P . Long Evans rats received intraocular injections of vehicle (Veh) OD and 250 ng of Norrin K86P (Nor) OS. Average retinal thickness was calculated from 24 measurements around the optic disk for each retina, before injection and then again three weeks post injection. Thickness was measured between the inner limiting membrane (ILM) and outer limiting membrane (OLM) of the neural retina. The retinal thickness values (mm) averaged from four different rats are shown; bars show standard deviation.
    Figure Legend Snippet: Retinal thickness before and after intraocular injection of Norrin K86P . Long Evans rats received intraocular injections of vehicle (Veh) OD and 250 ng of Norrin K86P (Nor) OS. Average retinal thickness was calculated from 24 measurements around the optic disk for each retina, before injection and then again three weeks post injection. Thickness was measured between the inner limiting membrane (ILM) and outer limiting membrane (OLM) of the neural retina. The retinal thickness values (mm) averaged from four different rats are shown; bars show standard deviation.

    Techniques Used: Injection, Membrane, Standard Deviation

    Mixed Rod–Cone ERG response of Long Evans rats. Long Evans rats received intraocular injections of vehicle (Veh) OD and 250 ng of Norrin K86P (Nor) OS. ( A ) Examples of Rod and mixed Rod–Cone ERG recordings from vehicle and contralateral Norrin K86P -injected eyes, 3 weeks post-injection. The left vertical marker indicates the light pulse flash at 0 milliseconds (ms). ( B ) Mixed Rod–Cone ERGs were measured, comparing vehicle-injected (OD) and Norrin-injected eyes, after dark adaptation. Average magnitudes of A-wave and B-wave (uV) are shown, using full-field white-light stimulation of 3.0 cd-sec/m 2 . Bars show standard deviation (N = 4 rats). No inhibition of Cone–Rod ERG response was detected in Norrin-treated versus vehicle-treated eyes.
    Figure Legend Snippet: Mixed Rod–Cone ERG response of Long Evans rats. Long Evans rats received intraocular injections of vehicle (Veh) OD and 250 ng of Norrin K86P (Nor) OS. ( A ) Examples of Rod and mixed Rod–Cone ERG recordings from vehicle and contralateral Norrin K86P -injected eyes, 3 weeks post-injection. The left vertical marker indicates the light pulse flash at 0 milliseconds (ms). ( B ) Mixed Rod–Cone ERGs were measured, comparing vehicle-injected (OD) and Norrin-injected eyes, after dark adaptation. Average magnitudes of A-wave and B-wave (uV) are shown, using full-field white-light stimulation of 3.0 cd-sec/m 2 . Bars show standard deviation (N = 4 rats). No inhibition of Cone–Rod ERG response was detected in Norrin-treated versus vehicle-treated eyes.

    Techniques Used: Injection, Marker, Standard Deviation, Inhibition

    Norrin-based protein sequences inserted into the pD454-MBP vector. Inserted sequences were added in frame following the MBP sequence. The resulting proteins were MBP N-terminal fusion proteins. ( A ) Nor-2 contained the mature human Norrin K86P , sequence underlined. The HRV3C protease cleavage site is shown in bold font. ( B ) Nor-w-Linker included extra linker sequences to improve the steric exposure of the HRV3C cleavage site. Three rigid helical linkers (blue font) and three flexible linkers (red font) are indicated. Two polypeptides identified by mass spectroscopy after electrophoresis of a Norrin-sized band (15 kDa) obtained after HRV3C protease treatment are highlighted with yellow.
    Figure Legend Snippet: Norrin-based protein sequences inserted into the pD454-MBP vector. Inserted sequences were added in frame following the MBP sequence. The resulting proteins were MBP N-terminal fusion proteins. ( A ) Nor-2 contained the mature human Norrin K86P , sequence underlined. The HRV3C protease cleavage site is shown in bold font. ( B ) Nor-w-Linker included extra linker sequences to improve the steric exposure of the HRV3C cleavage site. Three rigid helical linkers (blue font) and three flexible linkers (red font) are indicated. Two polypeptides identified by mass spectroscopy after electrophoresis of a Norrin-sized band (15 kDa) obtained after HRV3C protease treatment are highlighted with yellow.

    Techniques Used: Plasmid Preparation, Sequencing, Mass Spectrometry, Electrophoresis



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    rh norrin  (R&D Systems)
    93
    R&D Systems rh norrin
    Expression of tag-less <t>Norrin</t> <t>K86P</t> in BL21(DE3) cell inclusion body protein fractions. DISC-PAGE protein gel of inclusion body (IB) preparations from four separate batches are shown. IB material was dissolved and reduced by heating (100 °C × 10 min) in SDS sample buffer with 2-mercaptoethanol. Some dimeric and tetrameric bands can be formed during this treatment. Two different loading volumes (1 µL and 5 µL) were used for each of the four batches shown. Gels were stained with colloidal Coomassie blue. IB protein content was >98% recombinant Norrin K86P .
    Rh Norrin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rh norrin/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    rh norrin - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Expression of tag-less Norrin K86P in BL21(DE3) cell inclusion body protein fractions. DISC-PAGE protein gel of inclusion body (IB) preparations from four separate batches are shown. IB material was dissolved and reduced by heating (100 °C × 10 min) in SDS sample buffer with 2-mercaptoethanol. Some dimeric and tetrameric bands can be formed during this treatment. Two different loading volumes (1 µL and 5 µL) were used for each of the four batches shown. Gels were stained with colloidal Coomassie blue. IB protein content was >98% recombinant Norrin K86P .

    Journal: International Journal of Molecular Sciences

    Article Title: Development of Norrin-Based Protein Therapeutic for Activation of Norrin-Wnt Signaling in Human Retinal Endothelial Cells

    doi: 10.3390/ijms262311340

    Figure Lengend Snippet: Expression of tag-less Norrin K86P in BL21(DE3) cell inclusion body protein fractions. DISC-PAGE protein gel of inclusion body (IB) preparations from four separate batches are shown. IB material was dissolved and reduced by heating (100 °C × 10 min) in SDS sample buffer with 2-mercaptoethanol. Some dimeric and tetrameric bands can be formed during this treatment. Two different loading volumes (1 µL and 5 µL) were used for each of the four batches shown. Gels were stained with colloidal Coomassie blue. IB protein content was >98% recombinant Norrin K86P .

    Article Snippet: The cells were weaned to media containing no Hydrocortisone Hemisuccinate prior to stimulation with Norrin K86P or rh Norrin (R&D Systems, Minneapolis, MN, USA; 3014-NR).

    Techniques: Expressing, Staining, Recombinant

    Fundus and fluorescein angiography images before and after injection of Norrin K86P . Long Evans rats were imaged pre injection and 3 weeks post injection of 250 ng Norrin K86P . An example of the same Norin K86P -treated eye (OS) and vehicle-treated contralateral eye (OD) are shown. Brightfield fundus images are shown for each eye with their corresponding fluoresceine angiography image below. Angiography shows the perfused microvasculature of the neural retina (green). No impact on the retinal vasculature was noted for any eyes, whether Norin K86P - or vehicle-injected, across all rats tested. (N = 4). The same retinas were also analyzed for retinal thickness using SD-OCT.

    Journal: International Journal of Molecular Sciences

    Article Title: Development of Norrin-Based Protein Therapeutic for Activation of Norrin-Wnt Signaling in Human Retinal Endothelial Cells

    doi: 10.3390/ijms262311340

    Figure Lengend Snippet: Fundus and fluorescein angiography images before and after injection of Norrin K86P . Long Evans rats were imaged pre injection and 3 weeks post injection of 250 ng Norrin K86P . An example of the same Norin K86P -treated eye (OS) and vehicle-treated contralateral eye (OD) are shown. Brightfield fundus images are shown for each eye with their corresponding fluoresceine angiography image below. Angiography shows the perfused microvasculature of the neural retina (green). No impact on the retinal vasculature was noted for any eyes, whether Norin K86P - or vehicle-injected, across all rats tested. (N = 4). The same retinas were also analyzed for retinal thickness using SD-OCT.

    Article Snippet: The cells were weaned to media containing no Hydrocortisone Hemisuccinate prior to stimulation with Norrin K86P or rh Norrin (R&D Systems, Minneapolis, MN, USA; 3014-NR).

    Techniques: Injection

    Retinal thickness before and after intraocular injection of Norrin K86P . Long Evans rats received intraocular injections of vehicle (Veh) OD and 250 ng of Norrin K86P (Nor) OS. Average retinal thickness was calculated from 24 measurements around the optic disk for each retina, before injection and then again three weeks post injection. Thickness was measured between the inner limiting membrane (ILM) and outer limiting membrane (OLM) of the neural retina. The retinal thickness values (mm) averaged from four different rats are shown; bars show standard deviation.

    Journal: International Journal of Molecular Sciences

    Article Title: Development of Norrin-Based Protein Therapeutic for Activation of Norrin-Wnt Signaling in Human Retinal Endothelial Cells

    doi: 10.3390/ijms262311340

    Figure Lengend Snippet: Retinal thickness before and after intraocular injection of Norrin K86P . Long Evans rats received intraocular injections of vehicle (Veh) OD and 250 ng of Norrin K86P (Nor) OS. Average retinal thickness was calculated from 24 measurements around the optic disk for each retina, before injection and then again three weeks post injection. Thickness was measured between the inner limiting membrane (ILM) and outer limiting membrane (OLM) of the neural retina. The retinal thickness values (mm) averaged from four different rats are shown; bars show standard deviation.

    Article Snippet: The cells were weaned to media containing no Hydrocortisone Hemisuccinate prior to stimulation with Norrin K86P or rh Norrin (R&D Systems, Minneapolis, MN, USA; 3014-NR).

    Techniques: Injection, Membrane, Standard Deviation

    Mixed Rod–Cone ERG response of Long Evans rats. Long Evans rats received intraocular injections of vehicle (Veh) OD and 250 ng of Norrin K86P (Nor) OS. ( A ) Examples of Rod and mixed Rod–Cone ERG recordings from vehicle and contralateral Norrin K86P -injected eyes, 3 weeks post-injection. The left vertical marker indicates the light pulse flash at 0 milliseconds (ms). ( B ) Mixed Rod–Cone ERGs were measured, comparing vehicle-injected (OD) and Norrin-injected eyes, after dark adaptation. Average magnitudes of A-wave and B-wave (uV) are shown, using full-field white-light stimulation of 3.0 cd-sec/m 2 . Bars show standard deviation (N = 4 rats). No inhibition of Cone–Rod ERG response was detected in Norrin-treated versus vehicle-treated eyes.

    Journal: International Journal of Molecular Sciences

    Article Title: Development of Norrin-Based Protein Therapeutic for Activation of Norrin-Wnt Signaling in Human Retinal Endothelial Cells

    doi: 10.3390/ijms262311340

    Figure Lengend Snippet: Mixed Rod–Cone ERG response of Long Evans rats. Long Evans rats received intraocular injections of vehicle (Veh) OD and 250 ng of Norrin K86P (Nor) OS. ( A ) Examples of Rod and mixed Rod–Cone ERG recordings from vehicle and contralateral Norrin K86P -injected eyes, 3 weeks post-injection. The left vertical marker indicates the light pulse flash at 0 milliseconds (ms). ( B ) Mixed Rod–Cone ERGs were measured, comparing vehicle-injected (OD) and Norrin-injected eyes, after dark adaptation. Average magnitudes of A-wave and B-wave (uV) are shown, using full-field white-light stimulation of 3.0 cd-sec/m 2 . Bars show standard deviation (N = 4 rats). No inhibition of Cone–Rod ERG response was detected in Norrin-treated versus vehicle-treated eyes.

    Article Snippet: The cells were weaned to media containing no Hydrocortisone Hemisuccinate prior to stimulation with Norrin K86P or rh Norrin (R&D Systems, Minneapolis, MN, USA; 3014-NR).

    Techniques: Injection, Marker, Standard Deviation, Inhibition

    Norrin-based protein sequences inserted into the pD454-MBP vector. Inserted sequences were added in frame following the MBP sequence. The resulting proteins were MBP N-terminal fusion proteins. ( A ) Nor-2 contained the mature human Norrin K86P , sequence underlined. The HRV3C protease cleavage site is shown in bold font. ( B ) Nor-w-Linker included extra linker sequences to improve the steric exposure of the HRV3C cleavage site. Three rigid helical linkers (blue font) and three flexible linkers (red font) are indicated. Two polypeptides identified by mass spectroscopy after electrophoresis of a Norrin-sized band (15 kDa) obtained after HRV3C protease treatment are highlighted with yellow.

    Journal: International Journal of Molecular Sciences

    Article Title: Development of Norrin-Based Protein Therapeutic for Activation of Norrin-Wnt Signaling in Human Retinal Endothelial Cells

    doi: 10.3390/ijms262311340

    Figure Lengend Snippet: Norrin-based protein sequences inserted into the pD454-MBP vector. Inserted sequences were added in frame following the MBP sequence. The resulting proteins were MBP N-terminal fusion proteins. ( A ) Nor-2 contained the mature human Norrin K86P , sequence underlined. The HRV3C protease cleavage site is shown in bold font. ( B ) Nor-w-Linker included extra linker sequences to improve the steric exposure of the HRV3C cleavage site. Three rigid helical linkers (blue font) and three flexible linkers (red font) are indicated. Two polypeptides identified by mass spectroscopy after electrophoresis of a Norrin-sized band (15 kDa) obtained after HRV3C protease treatment are highlighted with yellow.

    Article Snippet: The cells were weaned to media containing no Hydrocortisone Hemisuccinate prior to stimulation with Norrin K86P or rh Norrin (R&D Systems, Minneapolis, MN, USA; 3014-NR).

    Techniques: Plasmid Preparation, Sequencing, Mass Spectrometry, Electrophoresis